Sepharose sp column torrent

Use a weak ion exchanger such as deae, cm or anx sepharose fast flow, if a strong ion exchanger substituted with q, s or sp does not give the required selectivity. Pdf q sepharose high performance and sp sepharose high. It has the stateofart sapfire 64bit solver to analyze extremely large and complex projects rapidly. Sp sepharose has also assisted with advancing industrial. Can one use sp sepharose rather than mono s for protein. Why my target protein is not eluted from a qsepharose column. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml. Application sp sepharose is used in protein chromatography, ion exchange chromatography and cation exchange media. Both remain charged and maintain high capacity over broad ph ranges. Supplied connector kit with hitrap column media properties sp sepharose high performance and q sepharose high performance are strong cation and strong anion exchangers respectively. With newly introduced spresults and spreporter modules and powered by the advanced and flexible graphical interface of new spsection module for creating and modifying irregular sections, spcolumn v6. For processscale applications, ge recommends axichrom columns.

A m e r s h a m b i o s c i e n c e s important user information please read these instructions carefully before using q sepharose fast flow media. Sp sepharose fast flow is compatible with two of these lines, tricorn and xk columns. Ion exchange chromatography columns and resins ge healthcare. The software supports nonlinear modeling techniques such as construction sequencing and time effects. Protein purification pouring and packing an agarose column. Wash the gel with 510 gel volumes of distilled water on a glass filter. Pdf q sepharose high performance sp sepharose high. Sp sepharose fast flow cation exchange chromatography. The columns have an advanced design and are constructed from precision bore borosilicate glass. Titrationcurveofdeaesepharosefastflow 8 71500964ag.

To modify these instructions for columns of different dimensions, refer to appendix a. The functional groups are coupled to the matrix via chemically stable ether linkages. Sp sepharose fast flow can also be packed in the other column platforms such as bpg and chromaflow. Cm sepharose fast flow deae sepharose fast flow q sepharose. What is the best way of packing sepharose cl6b matrix in a. Equipped with latest american aci 31814 and canadian csa a23. Use sepharose fast flow for capture or intermediate purification steps that require good resolution flows up to 300 cmh.

Pour the gel slurry into the column in one continuous motion. The protocol calls for sp sepharose and subsequently cm sepharose columns for purification of the protein. Can one use sp sepharose rather than mono s for protein purification. Availability in different prepacked hiprep and hitrap column formats plus. Protocols and tips in protein purification the university of sheffield. What is the best way of packing sepharose cl6b matrix in a column.

Sepharose high sp sepharose high performance q sepharose high performance, and sp sepharose high performance, are strong ion exchangers. Instructions71500964ag ionexchangeresins cmsepharosefastflow deaesepharosefastflow qsepharosefastflow spsepharosefastflow cm,deae. Sp sepharose has been used to study inhibitory proteins and peptides from the rhizomes of zingiberaceae plants as well as to study the subunit heterogeneity and molecular evolution of soybean basic 7s globulin. Sp sepharose fast flow, aqueous ethanol suspension, 45. Q sepharose high performance and sp sepharose high. A m e r s h a m b i o s c i e n c e s q sepharose fast flow.